Mac-2 antigen is therefore induced in macrophages only in response to specific differentiative signals. SDS-PAGE of -methionine-labeled Mac-2 shows that thioglycollate-elicited macrophages synthesize 10- to 30-fold more Mac-2 than other peritoneal macrophage subpopulations, whereas all types of peritoneal macrophages synthesize and express on their surfaces similar amounts of the Mac-1 antigen. Only 20% of peptone-elicited cells are weakly positive, whereas resident peritoneal macrophages and other macrophage elicited by Listeria monocytogenes, Con A, or LPS are greater than 98% negative. In contrast, thioglycollate-elicited macrophages are greater than 96% strongly positive for Mac-2. Cell suspensions from spleen, bone marrow, thymus, and peripheral lymph node are greater than 99% Mac-2- by immunofluorescent flow cytometry.
NOMACHINE MAC 2 MOUSE POINTERS PASSWORD
When the password is generated, select it using mouse cursor, copy and paste into the. Nonlymphoid tissues, such as lung, liver, heart, and brain, exhibit slight or no absorbing capacity. Click Set Password and enter new password for each user. Lymph node and thymus are negative, whereas spleen and bone marrow are weakly absorptive, probably due to stromal cells. Kidneys are also absorptive however, evidence is presented pointing to the nonspecificity of this absorption. Thioglycollate-elicited macrophages are strongly absorptive for 125I-labeled M3/38 MAb.
Saturation binding experiments show that thioglycollate-elicited macrophages express 1.7 X 10(5) Mac-2 sites/cell. Co-precipitation experiments show that antigenic determinants recognized by these two antibodies reside on the same molecular species, termed Mac-2, Mac-2, an antigen of 32,000 Mr, is synthesized by and expressed on the surface of thioglycollate-elicited macrophages as shown by -methionine and 125I labeling. Two monoclonal antibodies, M3/31 and M3/38, were obtained by fusion of mouse myeloma cells with rat spleen cells immunized to immunoadsorbent-purified macrophage glycoproteins.
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